2280

Fully automated 22 parameter CBC system

Excell22
Features Performance CD4 Testing Screens Brochure

Intended Use :

Top of the range Cell Counter for medium-sized laboratories
With fully automated CD4 and CD4% testing.


Features :

  • 22 parameters, 2 scattergrams and 2 histograms
  • Optional CD4 and CD4% also available
  • Short sample detection
  • Proprietary laser technology, 5 part WBC differential
  • Impedance technology for cell counting
  • State of the art patented platelet counting technique
  • 80 samples per hour
  • Sample size flexibility satisfies a wide range of end user requirements

Parameters :

WBC, Lymp#, Neut#, Mono#, Eos#, Baso#, Lymp%, Neut%, Mono%, Eos%, Baso%,
RBC, Hgb, Hct, MCV, MCH, MCHC, RDW,
Plt, MPV, Pct*, PDW*
(* - USA : for research only)
The system also displays :
2 x WBC scattergrams, Plt histogram, RBC histogram


Operation :

Scientific Principle :
Multi-dimensional optical scattering using stable laser diode light source to optimize the measurement of WBC differential
Electrical resistance for counting (WBC, RBC, Plt) and sizing (Plt,RBC)
Optical absorbance of cyanmethemoglobin for hemoglobin measurement (there is also an optional cyanide-free reagent).
Full CBC with 5 part differential in under one minute.

Sample Volumes :
Blood samples must be collected in EDTA tubes. Depending on how the 2280 is operated, the system requires :

Direct mode
Sample saver mode
Automatic sampler**
180 µL whole blood
80 µL whole blood
180 µL whole blood

An optional 30 position autosampler is available.
The Autosampler mixes, reads the barcode, pierces the tube cap and automatically aspirates the sample.

Dilution system :

High precision ceramic shear valve and computer controlled diluent dispenser. Automatic rinse inside and outside of the sample probe prevents carryover and reduces risk of transmission of blood borne pathogen to operators.


Sensors :

Laser diode illuminated flow cytometer with three, solid state sensors and one PMT tube for WBC differential.

WBC aperture 100 µm dia x 75 µm length
RBC/Plt aperture 78 µm dia x 55 µm length

The Plt/RBC channel features the patented "von Behrens" silencer that eliminates RBC recirculation which interferes with low Plt counts in conventional aperture designs.


System Software :

The 2280's easy to use software gives rapid access to all patient information including full blood counts, demographics and patient history. QC, calibration and reporting routines are all a few key strokes away.

Microsoft Windows XP™

Patient identification : Sample ID, PID (12 digits), name, first name, sex, DoB, comments
Patient data storage : 100,000 results with scattergrams & histograms
Report format : User selectable
Transfer protocols : ASTM or proprietary
Calibration : Automatic or manual
Selectable units : MKS, SI, SI modified, Chinese
Patient normal range : For up to 8 age ranges and user defined.
Patient action limits : user defined
Quality Control : A comprehensive onboard QC package allows optimal monitoring of system performance. 12 QC lots with 3 levels each. QC values can be loaded via keyboard or disk. QC data log with automatic calculation of mean, SD, CV and XB can be viewed or printed. Monthly Levey-Jennings plot. For comparison with other systems, QC results can be transferred for peer to peer analysis via floppy disk. Bull’s moving average.
Operation alerts : Full range of diagnostic alerts.
Maintenance: Programmable automatic startup and shutdown procedures. Maintenance log records routine maintenance operations such as startup, shutdown, reagent replacement and calibration.
Multi-language capability

Performance :

Precision :
  Level Units CV
WBC 8.0 x 10³ cells/µL < 1.5 %
RBC 5.0 x 106 cells/µL < 1.0 %
Hgb 16 g/dL < 1.0 %
MCV 90 fL < 1.0 % 
Plt 250 x 10³ cells/µL < 3.0 %
Linearity :
  Range Units Linearity
(whichever greater)
WBC 0.1 to 150.0 x 10³ cells/µL ± 0.2 or ± 2%
RBC 0.02 to 9.99 x 106 cells/µL ± 0.05 or ± 2%
Hgb 0.0 to 30 g/dL ± 0.2 or ± 2%
MCV 40 to 150 fl  
Plt 10 to 1000 x 10³ cells/µL ± 10 or ± 5.0%
Plt 10 to 2000 x 10³ cells/µL ± 15 or ± 7%

Automatic correction of Plt, RBC, MCV, MPV when plt and RBC interference detected


Dimensions :

Dimensions (analyzer) 42cm (h) x 58cm (w) x 58 cm (d)
16.5in (h) x 22.8in (w) x 22.8in (d)
Weight(analyzer) 32.5 Kg (72lb)
Operating conditions : Temperature : 15ºC to 32ºC
Humidity : 10% - 90% non-condensing
Voltage: 90 to 250 VAC
47 to 63 Hz
(no manual adjustment)

Ordering Info

Part Code Description
INSTRUMENTATION
BAR-9000-221 2280 Hematology analyzer with computer & barcode reader
BAR-9001-221 2280 Hematology analyzer without computer & barcode reader
DIL-9032-130 EX-SAM 30 autosampler with barcode reader
DIL-9031-130 EX-SAM 30 autosampler without barcode reader
2280 CONSUMABLES
RA-1720 EX-ISO (20L) Isotonic Diluent
RA-9500 EX-LYSE (0.5L)
RA-9500CF EX-LYSE - Cyanide free (0.5L)
RA-004C EX-ZYME (10L) Clean
RA-6010 EX-FLO (10L) Sheath Reagent
5 PART WHITE CELL DIFFERENTIAL QUALITY CONTROL
EXA-339+ EX-TROL + tri-level + 1 EX-CAL + (L,N,H 9x3mL of each control)
EXA-3L+ EX-TROL+ Low (1x3mL)
EXA-3N+ EX-TROL+ Normal (1x3mL)
EXA-3H+ EX-TROL+ High (1x3mL)

CD4 Testing :

Drew is proud to offer a unique, fully automated, closed tube sampling, whole blood method for CD4 and CD4% testing.

The CD4% test is crucial in optimising anti-retroviral therapy provided to pediatric and adult HIV/AIDS patients.

  The 2280/CD4% Method Other CD4 Only Methods Benefit
Sample loading Load the blood tube directly into the instrument Remove cap from blood tube
Pipette blood into test tube
No exposure to potential bio-hazardous material
Pre-analytical stages None
Instrument performs all pipetting, incubating and sample handling steps automatically
Add reagents to test tube
Allow test tube to incubate offline
Load the test tube into the instrument
No exposure to potential bio-hazardous material
No accuracy errors introduced by manual sample handing
Result interpretation Fully automatic data analysis and result generation Operator interprets cytogram and manually gates clusters to allow enumeration of cells No inaccuracies due to manual gating of cell clusters
Reagent storage Reagents are stored with the instrument Reagents stored in refrigerator No refrigeration required
Supplementary equipment No pipettes are required Calibrated pipettes and pipette tips must be available Reduced costs
Quick training Operator trained within a few hours Operator attends formal training over a few days Your can produce accurate results from day one

 

This test is available for the first time in a completely automatic format with no predetermination, sample manipulation or post measurement operator involvement.

An operator with brief training is able to perform the testing protocol by simply loading the automatic sampling module and selecting the analyse command. Once the sample is loaded the user is entirely free to perform other laboratory tasks.

On completion of the analysis a CD4 and CD4% report together with a comprehensive 22 parameter hemogram is available for reporting to the ordering physician.

Comprehensive results without Biohazard exposure
The 2280 system uses an advanced flow cytometer. This has eliminated the need for fluorescence when immunopheno-typing blood cells, such as the T-Helper (CD4+) subset of lymphocytes. The 2280 cytograms are automatically created and populations characterized with a simultaneous complete blood cell count, without any sample or software manipulation or instrument operator intervention. The operator simply loads the closed whole blood EDTA patient sample, and the 2280 system automatically performs all remaining steps. Reagent addition, mixing, incubation and analysis are all completed without any operator participation, and thus without any biohazard exposure.

High throughput and Ease of use
The ease of use of the 2280 system allows for independent laboratory operation within the day of training and installation. The 2280 reagent pack does not require refrigeration and is free of any restrictions of ambient light exposure. This is a first for immunophenotyping reagents. Experienced flow cytometry operators have asked for all these methodology improvements and the 2280 is now offering them in a fully automated format. The 2280 high throughput sample processing meets the needs and demands of the busiest laboratories.

Reliable high technology
The 2280 uses gold nano-particles, conjugated to CD4 monoclonal antibodies, to selectively label cell surface antigens on the CD4 positive lymphocytes. With this method it is possible to gain sensitivity equal to fluorescence without all the limitations of fluorescence. Stable, factory fixed light scatter detectors make the critical cell measurements necessary to accurately perform a five part differential and together with the unique light scatter signature provided by gold nanoparticles measure CD4 positive lymphocytes. The 2280 light scatter detectors and software create state of the art cytograms for automatic sample analysis.

CBC Screen :

CBC Screen

CD4 Screen :

CD4 Screen

Brochure :

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